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( a ) Type I IFN activity in plasma from 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=7 mice per group). (b-d) Ifnb1 transcripts in peripheral blood monocytes (b) , bone marrow (c) and splenocytes (d) of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice. ( b, c : n=3-4 mice per group) ( d : n=8-12 mice per group) (e) Cell counts of <t>CD11b</t> + Ly6C + high inflammatory monocytes in blood of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice. (n=8 mice per group). (f) Cell surface expression of the activation marker CD86 on the inflammatory monocytes in blood of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=8 mice per group). (g-h) Heatmap showing mean of differently expressed type I-IFN/inflammatory ( g ) and senescence ( h ) genes in splenocytes of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=3 mice per group). (i) SA-β-Gal activity in iWAT of Lrrk2 WT and Lrrk2 GoF mice. (j) Quantification of SA-β-Gal activity in iWAT of Lrrk2 WT and Lrrk2 GoF mice. (n=3 mice per group). Statistical tests: one-way ANOVA followed by Tukey’s post hoc test ( a-f, j ). Error bars represent mean ± SEM.
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( a ) Type I IFN activity in plasma from 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=7 mice per group). (b-d) Ifnb1 transcripts in peripheral blood monocytes (b) , bone marrow (c) and splenocytes (d) of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice. ( b, c : n=3-4 mice per group) ( d : n=8-12 mice per group) (e) Cell counts of <t>CD11b</t> + Ly6C + high inflammatory monocytes in blood of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice. (n=8 mice per group). (f) Cell surface expression of the activation marker CD86 on the inflammatory monocytes in blood of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=8 mice per group). (g-h) Heatmap showing mean of differently expressed type I-IFN/inflammatory ( g ) and senescence ( h ) genes in splenocytes of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=3 mice per group). (i) SA-β-Gal activity in iWAT of Lrrk2 WT and Lrrk2 GoF mice. (j) Quantification of SA-β-Gal activity in iWAT of Lrrk2 WT and Lrrk2 GoF mice. (n=3 mice per group). Statistical tests: one-way ANOVA followed by Tukey’s post hoc test ( a-f, j ). Error bars represent mean ± SEM.
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( a ) Type I IFN activity in plasma from 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=7 mice per group). (b-d) Ifnb1 transcripts in peripheral blood monocytes (b) , bone marrow (c) and splenocytes (d) of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice. ( b, c : n=3-4 mice per group) ( d : n=8-12 mice per group) (e) Cell counts of CD11b + Ly6C + high inflammatory monocytes in blood of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice. (n=8 mice per group). (f) Cell surface expression of the activation marker CD86 on the inflammatory monocytes in blood of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=8 mice per group). (g-h) Heatmap showing mean of differently expressed type I-IFN/inflammatory ( g ) and senescence ( h ) genes in splenocytes of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=3 mice per group). (i) SA-β-Gal activity in iWAT of Lrrk2 WT and Lrrk2 GoF mice. (j) Quantification of SA-β-Gal activity in iWAT of Lrrk2 WT and Lrrk2 GoF mice. (n=3 mice per group). Statistical tests: one-way ANOVA followed by Tukey’s post hoc test ( a-f, j ). Error bars represent mean ± SEM.

Journal: bioRxiv

Article Title: Aging-associated endolysosomal decline drives inflammaging and neurodegeneration through the STING-IFN-I axis

doi: 10.64898/2026.03.15.711864

Figure Lengend Snippet: ( a ) Type I IFN activity in plasma from 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=7 mice per group). (b-d) Ifnb1 transcripts in peripheral blood monocytes (b) , bone marrow (c) and splenocytes (d) of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice. ( b, c : n=3-4 mice per group) ( d : n=8-12 mice per group) (e) Cell counts of CD11b + Ly6C + high inflammatory monocytes in blood of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice. (n=8 mice per group). (f) Cell surface expression of the activation marker CD86 on the inflammatory monocytes in blood of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=8 mice per group). (g-h) Heatmap showing mean of differently expressed type I-IFN/inflammatory ( g ) and senescence ( h ) genes in splenocytes of 3 and 12 months old Lrrk2 WT and Lrrk2 GoF mice (n=3 mice per group). (i) SA-β-Gal activity in iWAT of Lrrk2 WT and Lrrk2 GoF mice. (j) Quantification of SA-β-Gal activity in iWAT of Lrrk2 WT and Lrrk2 GoF mice. (n=3 mice per group). Statistical tests: one-way ANOVA followed by Tukey’s post hoc test ( a-f, j ). Error bars represent mean ± SEM.

Article Snippet: Followed by isolation of brain microglia cells via magnetic isolation using CD11b positive Microbeads (Milteny biotech, #130-049-601).

Techniques: Activity Assay, Clinical Proteomics, Expressing, Activation Assay, Marker